List of animated works with LGBT characters

This is a list of animation works with LGBTQ+ characters. This list includes gay, lesbian, bisexual and transgender fictional characters in animated feature films, animated short films, animated shows, and anime.

History
Harry Benshoff and Sean Griffin write that animation has always "hint[ed] at the performative nature of gender" such as when Bugs Bunny puts on a wig and a dress, he is a rabbit in drag as a human male who is in drag as a female.

Within the Japanese anime and manga, yaoi is the tradition of representing same-sex male relationships in materials that are generally created by women artists and marketed mostly for Japanese girls while the genre known as yuri focuses on relationships between women.

Some LGBT characters in animation are derived from graphic art works and video games.